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Gene expression analysis of pancreatic cell lines reveals genes overexpressed in pancreatic cancer
2014-03-04
[Electronic ed.]
4519974-7
Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden
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Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, Dresden
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Background: Pancreatic cancer is one of the leading causes of cancer-related death. Using DNA gene expression analysis based on a custom made Affymetrix cancer array, we investigated the expression pattern of both primary and established pancreatic carcinoma cell lines. Methods: We analyzed the gene expression of 5 established pancreatic cancer cell lines (AsPC-1, BxPC-3, Capan-1, Capan-2 and HPAF II) and 5 primary isolates, 1 of them derived from benign pancreatic duct cells. Results: Out of 1,540 genes which were expressed in at least 3 experiments, we found 122 genes upregulated and 18 downregulated in tumor cell lines compared to benign cells with a fold change > 3. Several of the upregulated genes (like Prefoldin 5, ADAM9 and E-cadherin) have been associated with pancreatic cancer before. The other differentially regulated genes, however, play a so far unknown role in the course of human pancreatic carcinoma. By means of immunohistochemistry we could show that thymosin [β-10 (TMSB10), upregulated in tumor cell lines, is expressed in human pancreatic carcinoma, but not in non-neoplastic pancreatic tissue, suggesting a role for TMSB10 in the carcinogenesis of pancreatic carcinoma. Conclusion: Using gene expression profiling of pancreatic cell lines we were able to identify genes differentially expressed in pancreatic adenocarcinoma, which might contribute to pancreatic cancer development.
Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG-geförderten) Allianz- bzw. Nationallizenz frei zugänglich.
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Immunhistochemie, Zellkultur, Krebs, Bauchspeicheldrüse, Pankreas, Thymosin, Primärisolate
Pancreas, Cancer, Cell line, Primary isolates, Microarray, Thymosin, Immunohistochemistry
urn:nbn:de:bsz:14-qucosa-136495
406071578
10.1159/000086537
Karger
pbl
Karger, Basel
Ingo
Alldinger
aut
Dag
Dittert
aut
Matthias
Peiper
aut
Alberto
Fusco
aut
Gennaro
Chiappetta
aut
Eike
Staub
aut
Matthias
Löhr
aut
Ralf
Jesenofsky
aut
Gustavo
Baretton
aut
Detlef
Ockert
aut
Hans-Detlev
Saeger
aut
Robert
Grützmann
aut
Christian
Pilarsky
aut
eng
2005
http://dx.doi.org/10.1159/000086537
Volltext des Artikels, der zuerst in der Zeitschrift "Pancreatology" des Karger-Verlages erschienen ist. Die Zeitschrift erscheint seit 2012 beim Elsevier-Verlag.
Pancreatology 2005;5:370−379, ISSN: 1424-3903
born digital
SJ
susan.jankowski@slub-dresden.de
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