tud
Initiation of blood coagulation - Evaluating the relevance of specific surface functionalities using self assembled monolayers
2010-07-05
[Electronic ed.]
4519974-7
Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden
prv
Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, Dresden
Mathematik und Naturwissenschaften
Professur für Biofunktionelle Polymermaterialien
female
Dresden
The surface of biomaterials can induce contacting blood to coagulate, similar to the response initiated by injured blood vessels to control blood loss. This poses a challenge to the use of biomaterials as the resulting coagulation can impair the performance of hemocompatible devices such as catheters, vascular stents and various extracorporeal tubings [1], what can moreover cause severe host reactions like embolism and infarction.
Biomaterial induced coagulation processes limit the therapeutic use of medical products, what motivates the need for a better understanding of the basic mechanisms leading to this bio-incompatibility [2] in order to define modification strategies towards improved biomaterials [3]. Several approaches for the enhancement of hemocompatible surfaces include passive and active strategies for surface modifications. The materials’
chemical-physical properties like surface chemistry, wettability and polarity are parameters of passive modification approaches for improved hemocompatibility and are the focus of the present work.
In the present study self assembled monolayers with different surface functionalities (-COOH, -OH, -CH3) were applied as well as two-component-layers with varying fractions of these, as they allow a defined graduation of surface wettability and charge.
The ease of control over these parameters given by these model surfaces enables the evaluation of the influence of specific surface-properties on biological responses.
To evaluate the effects of different surface chemistry on initial mechanisms of biomaterial induced coagulation, the surfaces were incubated with protein solution, human plasma, blood cell fractions or fresh heparinised human whole blood. Indicative hemocompatibility parameters were subsequently analysed focusing on protein adsorption, coagulation activation, contact activation (intrinsic/ enhancer pathway), impact of tissue factor (extrinsic/ activator pathway) and cellular systems (blood
platelets and leukocytes).
570
WW 8960
Blood coagulation, cell activation, self assembled monolayers, hemocompatibility
Blutgerinnung, Zellaktivierung, Self assembled monolayer, Hämokompatibilität
urn:nbn:de:bsz:14-qucosa-38768
325667810
Technische Universität Dresden
dgg
Technische Universität Dresden
Marion
Fischer
1979-10-16
aut
Carsten
Werner
Prof. Dr.
dgs
rev
Brigitte
Voit
Prof. Dr.
rev
eng
2010-05-06
2010-06-24
born digital
Marion Fischer
motteimnet@web.de
doctoral_thesis