ubc Biocatalytic Production, Preparation and Characterization of Large-ring Cyclodextrins 2009-03-04 [Electronic ed.] prv Universitätsbibliothek Chemnitz Universitätsbibliothek Chemnitz, Chemnitz Fakultät für Maschinenbau male Kelantan, Malaysia male male male male 6 – CD₈. LR-CD may offer unique molecular recognition properties and can be produced biocatalytically from starch using cyclodextrin glucanotransferase (CGTase, E.C. 2.4.1.19) in a short reaction time. LR-CD were isolated from glucose, CD₆ – CD₈ and other compounds by complexation of CD₆ – CD₈ as well as precipitation techniques. The yield of LR-CD (degree of polymerization from 9 to 21) was optimized using central composite design. Addition of polar organic solvents to the synthesis resulted in higher yields of LR-CD. LR-CD composed of 9 to 21 glucose units were successfully separated using reversed-phase of ODS-AQ chromatography and normal-phase of polyamine II chromatography. Maintaining optimized reaction conditions aided in a high yield of CD₉; it could be separated with reasonable yield using a single step of polyamine II chromatography. A co-grinding method helped to obtain higher solubilization levels of glibenclamide, vitamin A acetate and vitamin D₃ in CD₁₃, CD₁₀ and CD₁₁, respectively when compared to other CD. Vitamin K₁ was solubilized in distilled water with CD₆ – CD₁₃ using a co-precipitation method. When compared with other CD, CD₉ was seen to be the best solubilizer. The analysis of complexes using ESI MS showed spironolactone and glibenclamide complexed with CD₉ and CD₁₃, respectively.]]> 570 Bacillus macerans Molekulare Erkennung Large-ring cyclodextrins (LR-CD) central composite design co-grinding co-precipitation cyclodextrin glucanotransferase (CGTase) host-guest complexation molecular recognition supramolecular chemistry urn:nbn:de:bsz:ch1-200900431 Technische Universität Chemnitz dgg Technische Universität Chemnitz, Chemnitz Mohd Noriznan Mokhtar 1977-08-10 aut Wolfgang Zimmermann Prof. Dr. dgs rev Bernd Platzer Prof. Dr.-Ing. rev Norbert Sträter Prof. Dr. rev eng 2008-10-06 2009-01-26 born digital doctoral_thesis